Laboratory Practice Record 2023-07-01 11:30-17:30
Preparation of NB culture medium
Preparation of NB culture medium
NB medium is suitable for culturing various bacteria (1L)
The main materials are:
Peptone 10g
NaCl 5g
Beef paste 3g
Agar 20g
Put the prepared culture medium into a flask, seal it, and place it in an autoclave at 121 degrees Celsius for 25 minutes to sterilize.
After sterilization is completed, pour it into a petri dish and wait for solidification. Pay attention to prevent contamination during the process.